Molecular Cloning and characterization of GAPDH gene in tomato plants开题报告

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1. 研究目的与意义、国内外研究现状（文献综述）

Glyceraldeyde-3-phosphate dehydrogenase encodes a protein which has been identified as a moonlighting protein based on its ability to perform mechanically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism. In this project PCR is being used in order to analyse the qualification of messenger RNA sequences by either absolute or relative analysis methods, this is a sensitive method for the quantification of gene expression and specific quantification errors are easily compounded by any variation in the starting material between samples, such errors will compromise the accuracy of quantification and lead to misinterpretation of the results if they are not corrected.

2. 研究的基本内容和问题

research objectives

-defining and understanding molecular cloning and gapdh in details

-outline and describe the steps of the process used to clone gapdh in tomato plants .

3. 研究的方法与方案

1.the first method that i will use is the extraction of plant tissue rna in which a particular amount/ weight of the tissue will be enough to continue with the process.

2.reverse transcription is the second method

3.the third one is pcrand also pcr purification which will insure that i measure accurate concentration and the purity of the rna.

4. 研究创新点

-The use of a Mega6 system to help me with phylogenetic tree, which helps me to understand the evolutionary relationship between GAPDH and a tomato plant.

5. 研究计划与进展

my research plan for this project is as follows;

week 1

1.extraction of the plant tissue rna -which will take about 5 days