乌饭子多糖提取工艺优化及活性研究开题报告

1. 研究目的与意义

乌饭子，原名南烛，别名染菽、乌饭树、米饭树、乌饭叶等，拉丁文名为vaccinium bracteatum thunb。

乌饭子属于杜鹃花科、越桔属常绿灌木或小乔木，总状花序顶生和腋生，有多数花，序轴密被短柔毛稀无毛；熟时紫黑色，外面通常被短柔毛、稀无毛。

花期6-7月，果期8-10月。

2. 研究内容和预期目标

本论文研究主要内容如下：（1）乌饭子粉末原料制备，原料干燥和粉碎处理。

（2）对乌饭子进行超声时间、超声提取温度、液料比和超声功率的单因素试验，得到基本的提取条件。

（3）利用单因素试验结果，选取合适条件因素进行响应面优化实验，得到优化的乌饭子多糖提取工艺条件组合，并对其进行验证。

3. 研究的方法与步骤

研究方法：用乙酸乙酯萃取乌饭子原料；按照单因素设计原则进行试验；对料液进行高速冷冻离心机离心；对上清液进行旋蒸浓缩；对提取液用抽滤装置进行抽滤；对粗多糖采用真空冷冻干燥机进行冷冻干燥；傅里叶变换红外光谱法测定乌饭子多糖结构。

实验步骤：（1）乌饭子原料低温干燥至恒重后用干粉粉碎机粉碎，过60目筛后密封保存，备用。

（2）用乙酸乙酯萃取乌饭子原料，快速烘干至恒重，保存备用。

4. 参考文献

[1] 于莲, 张俊婷,马淑霞. 山药多糖提取工艺优化及其抗菌活性研究[J]. 中成药, 2014,36(6): 1194-1198.[2] Chen H G, Zhou X, Zhang J Z. Optimization of enzyme assisted extraction of polysaccharides from Astragalus membranaceus. Carbohydrate Polymers, 2014,111:567575.[3] Lia P, Zhou L G, MouY, et al. Extraction optimization of polysaccharide from Zanthoxy lumbungeanum using RSM and its antioxidant activity[J]. Inter- national Journal of Biological Macromolecules, 2015,72:1927.[4] Nour V, Trandafir I, Cosmulescu S. Antioxidant capacity, phenolic compounds and min- erals content of blackcurrant (Ribes nigrum L.) leaves as influenced by harvesting date and extraction method[J]. Industrial Crops and Products, 2014, 53:133-139.[5] Deng Q F, Zhou X, Chen H G. Optimization of enzyme assisted extraction of Fructus Mori polysaccharides and its activities on antioxidant and alcohol dehydrogenase[J]. Carbohydrate Polymers , 2014,111:775782.[6] Yang S, Jin L, Ren X D, et al. Optimization of fermentation process of Cordyceps militaris and antitumor activities of polysaccharides in vitro[J]. Journal of food and drug analsis,2014, 1-9.[7] Blanco-Pascual, Montero M P, Gmez-Guilln M C. Antioxidant film development from unrefined extracts of brown seaweeds Laminaria digitata and Ascophyllum nodosum[J]. Food Hydrocolloids, 2014, 37:100-110.[8] 王晓阳,唐琳,赵垒. 响应面法优化刺槐花多酚的超声提取工艺[J]. 2011, 32( 2):66-70. [9] Liu Y, Gong G L, Zhang J,et al. Response surface optimization of ultrasound -assisted enzymaticextraction polysaccharides from Lycium barbarum. [J]. Carbohydrate Polymers, 2014,110:278284.[10] Zhang G W, He L, Hu M M. Optimized ultrasonic-assisted extraction of flavor noids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro[J]. Innovative Food Science and Emerging Technologies, 2011, 12:18-25.[11] Shen S A, Chen D J, Li X, et al. Optimization of extraction process and antioxi -dant activity ofpolysaccharides from leaves of Paris polyphylla[J]. Carbo- hydrate Polymers, 2014, 104:8086.[12] 徐翠翠,孔浩,郭庆梅等. 响应面法优化孔石莼多糖提取工艺[J]. 中国海洋药物, 2014,33(5): 37-42.[13] Shang Y F, Kim S M, Um B H. Optimisation of pressurised liquid extraction of antioxidants from black bamboo leaves[J]. Food Chemistry, 2014,154:164 -170.[14] 安红钢,林敏,任雪峰,等. 茄蒂黄酮提取工艺及抗氧化性研究[J]. 中成药，2011,33(11): 1900-1904.[15] ZhangT L, Tua ZC, YuanT, et al. Solvent optimization, antioxidant activity, and chemicalcharacterization of extracts from Artemisia selengnesis TurczLu [J]. Industrial Crops and Products, 2014,56:223230.[16] Zhang Z S, Wang X M, Zhao M X, et al. Optimization of polysaccharides extraction from Clematis huchouensis Tamura and its antioxidant activity [J]. Carbohydrate Polymers , 2014,111:762767.

5. 计划与进度安排

本论文的各阶段安排为：1、2022-12-22～2022-03-20 接受任务、查阅和翻译文献、撰写及完成开题报告；2、2022-03-21～2022-04-11 原材料处理，对乌饭子进行超声时间、超声提取温度、液料比和超声功率的单因素试验，得到基本的提取条件；3、2022-04-12～2022-05-13 利用单因素试验结果，选取合适条件因素进行响应面优化实验，得到优化的乌饭子多糖提取工艺条件组合，并对其进行验证。

4、2022-05-14～2022-06-02对所得多糖进行进行初步抗氧化活性测定及多糖结构进行初步判定。

5、2022-06-03～2022-06-26 撰写毕业论文及论文答辩。